BIO 200 Lab 5, Spring 2016
American University of Beirut · Diversity of Life · 1 page
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Biology 200 Annex to Enzymes Experiments
In all sets of experiments monitor color change after 5 min
1.Enzyme Reactions
A 10ml water + 10 drops enzyme+10 drops substrate.
b. 10ml water +10 drops substrate.
c. 10ml water + 10 drops enzyme+10 drops sucrose.
2. Temperature
a. 10ml water + 10 drops enzyme+10 drops substrate. 0°C
b. 40°C; 60, 100
3. Concentration of enzyme
a. 10ml water + 3 drops enzyme+10 drops substrate.
b. 10ml water + 9 drops enzyme+10 drops substrate.
c. 10ml water + 27 drops enzyme+10 drops substrate.
4. Concentration of substrate
a. 10ml water + 10 drops enzyme+3 drops substrate.
b. 10ml water + 10 drops enzyme+ 9 drops substrate.
c. 10ml water + 10 drops enzyme+ 27 drops substrate.
5. Effect of pH
a. 10ml water (pH 3) + 10 drops enzyme+10 drops substrate.
b. 10ml water (pH 5) + 10 drops enzyme+10 drops substrate.
c. 10ml water (pH 7) + 10 drops enzyme+10 drops substrate.
d. 10ml water (pH 9) + 10 drops enzyme+10 drops substrate.
6. Inhibitors A. PTU
a. 10ml water + 10 drops enzyme+10 drops substrate+ 1 drop PTU.
b. 10ml water + 10 drops enzyme+10 drops substrate + 10 drops PTU.
c. 10ml water + 10 drops enzyme+10 drops substrate+ 20 drops PTU.
B. Tyrosine
d. 10ml water + 10 drops enzyme+10 drops substrate+ 1 drop tyrosine.
e. 10ml water + 10 drops enzyme+10 drops substrate+ 10 drop tyrosine.
f. 10ml water + 10 drops enzyme+10 drops substrate+ 20 drop tyrosine.